mbd3 overexpression plasmids Search Results


mbd3 overexpression plasmids  (Addgene inc)
93
Addgene inc mbd3 overexpression plasmids
Sequences of primers used for qPCR
Mbd3 Overexpression Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mbd3 overexpression plasmids/product/Addgene inc
Average 93 stars, based on 1 article reviews
mbd3 overexpression plasmids - by Bioz Stars, 2026-04
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control fuw m2rtta  (Addgene inc)
96
Addgene inc control fuw m2rtta
Sequences of primers used for qPCR
Control Fuw M2rtta, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/control fuw m2rtta/product/Addgene inc
Average 96 stars, based on 1 article reviews
control fuw m2rtta - by Bioz Stars, 2026-04
96/100 stars
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prl-tk internal control vector  (Promega)
90
Promega prl-tk internal control vector
Sequences of primers used for qPCR
Prl Tk Internal Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prl-tk internal control vector/product/Promega
Average 90 stars, based on 1 article reviews
prl-tk internal control vector - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


Sequences of primers used for qPCR

Journal: American Journal of Translational Research

Article Title: Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells

doi:

Figure Lengend Snippet: Sequences of primers used for qPCR

Article Snippet: Mbd3 overexpression in mES The Mbd3 overexpression plasmids, FUW-Mbd3 (#52356) and control FUW-M2rtTA (#20342), were purchased from Addgene.

Techniques:

Mbd3 overexpression induced cellular apoptosis. A. Overexpression of Mbd3 RNA levels was achieved by transfection with the FUW-Mbd3 plasmid in fifth-passage mES. The FUW-M2rtTA plasmid and the PKCi group were used as controls. B. Mbd3 protein expression was evaluated by Western blot in fifth-passage mES (upper panel). Quantitative density analysis showed that Mbd3 overexpression resulted in its increased protein levels compared with the PKCi control (lower panel). C. Mbd3 overexpression significantly reduced the total number of fifth-passage mES. D. CCK8 assay revealed that Mbd3 overexpression decreased mES cell viability. E. Apoptosis was evaluated by Annexin V staining in fifth-passage mES. The thick arrow indicates cell nuclei (blue), and the thin arrow indicates apoptotic cells (red). Scale bar =100 μm (left panel). The apoptosis rate was significantly increased with Mbd3 overexpression (right panel). F. Bax, Bcl-2, and β-actin protein levels were evaluated by Western blot in fifth-passage mES (left panel). Quantitative density analysis showed similar levels of Bax in all three groups (middle panel) and significantly reduced Bcl-2 levels with Mbd3 overexpression (right panel). G. Mbd3 overexpression increased the expression of Bax, Bim, Trail, Fasl, and caspase 3 RNA, but reduced the expression of Bcl-2 RNA, in fifth-passage mES. The data are represented as mean ± SD (n=3). *P<0.05, **P<0.01.

Journal: American Journal of Translational Research

Article Title: Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells

doi:

Figure Lengend Snippet: Mbd3 overexpression induced cellular apoptosis. A. Overexpression of Mbd3 RNA levels was achieved by transfection with the FUW-Mbd3 plasmid in fifth-passage mES. The FUW-M2rtTA plasmid and the PKCi group were used as controls. B. Mbd3 protein expression was evaluated by Western blot in fifth-passage mES (upper panel). Quantitative density analysis showed that Mbd3 overexpression resulted in its increased protein levels compared with the PKCi control (lower panel). C. Mbd3 overexpression significantly reduced the total number of fifth-passage mES. D. CCK8 assay revealed that Mbd3 overexpression decreased mES cell viability. E. Apoptosis was evaluated by Annexin V staining in fifth-passage mES. The thick arrow indicates cell nuclei (blue), and the thin arrow indicates apoptotic cells (red). Scale bar =100 μm (left panel). The apoptosis rate was significantly increased with Mbd3 overexpression (right panel). F. Bax, Bcl-2, and β-actin protein levels were evaluated by Western blot in fifth-passage mES (left panel). Quantitative density analysis showed similar levels of Bax in all three groups (middle panel) and significantly reduced Bcl-2 levels with Mbd3 overexpression (right panel). G. Mbd3 overexpression increased the expression of Bax, Bim, Trail, Fasl, and caspase 3 RNA, but reduced the expression of Bcl-2 RNA, in fifth-passage mES. The data are represented as mean ± SD (n=3). *P<0.05, **P<0.01.

Article Snippet: Mbd3 overexpression in mES The Mbd3 overexpression plasmids, FUW-Mbd3 (#52356) and control FUW-M2rtTA (#20342), were purchased from Addgene.

Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Western Blot, Control, CCK-8 Assay, Staining

Mbd3 knockdown partially reversed the cellular apoptosis induced by PKCi removal. A. Mbd3 RNA expression levels were increased in third-passage mES when PKCi was removed for 48 h from the culture medium, but they were only partially decreased with Mbd3 knockdown. B. Mbd3 protein levels were assessed by Western blot in third-passage mES (upper panel). Quantitative density analysis showed a significant increase in Mbd3 upon PKCi removal, and a partial reduction was observed with Mbd3 knockdown. C. The total number of third-passage mES was dramatically decreased upon PKCi removal and increased with Mbd3 knockdown, but it was still lower than that of the PKCi group. D. Cell viablility was improved by Mbd3 knockdown. E. Cellular apoptosis was assessed by Annexin V staining in third-passage mES. The thick arrow indicates cell nuclei (blue), and the thin arrow indicates apoptotic cells (red). Scale bar =100 μm (left panel). The apoptosis rate was significantly increased upon PKCi removal, but it was partially reduced with Mbd3 knockdown (right panel). F. Bax, Bcl-2, and β-actin levels were evaluated by Western blot in third-passage mES with Mbd3 knockdown (left panel). Quantitative density analysis showed increased levels of Bax after PKCi removal, but only partially reduced levels were observed with Mbd3 knockdown (middle panel). Bcl-2 expression was reduced upon PKCi removal, and similar levels were observed with Mbd3 knockdown (right panel). G. PKCi removal resulted in increased expression levels of Bax, Bim, Trail, Fasl, and caspase 3 RNA, with reduced Bcl-2 levels, in third-passage mES. Mbd3 knockdown resulted in partially reduced Bax, Bim, Trail, and caspase 3 RNA levels, unchanged Fasl levels, and increased Bcl-2 levels. The data are represented as mean ± SD (n=3). The letters a, b, c indicate significant differences among the groups (P<0.05).

Journal: American Journal of Translational Research

Article Title: Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells

doi:

Figure Lengend Snippet: Mbd3 knockdown partially reversed the cellular apoptosis induced by PKCi removal. A. Mbd3 RNA expression levels were increased in third-passage mES when PKCi was removed for 48 h from the culture medium, but they were only partially decreased with Mbd3 knockdown. B. Mbd3 protein levels were assessed by Western blot in third-passage mES (upper panel). Quantitative density analysis showed a significant increase in Mbd3 upon PKCi removal, and a partial reduction was observed with Mbd3 knockdown. C. The total number of third-passage mES was dramatically decreased upon PKCi removal and increased with Mbd3 knockdown, but it was still lower than that of the PKCi group. D. Cell viablility was improved by Mbd3 knockdown. E. Cellular apoptosis was assessed by Annexin V staining in third-passage mES. The thick arrow indicates cell nuclei (blue), and the thin arrow indicates apoptotic cells (red). Scale bar =100 μm (left panel). The apoptosis rate was significantly increased upon PKCi removal, but it was partially reduced with Mbd3 knockdown (right panel). F. Bax, Bcl-2, and β-actin levels were evaluated by Western blot in third-passage mES with Mbd3 knockdown (left panel). Quantitative density analysis showed increased levels of Bax after PKCi removal, but only partially reduced levels were observed with Mbd3 knockdown (middle panel). Bcl-2 expression was reduced upon PKCi removal, and similar levels were observed with Mbd3 knockdown (right panel). G. PKCi removal resulted in increased expression levels of Bax, Bim, Trail, Fasl, and caspase 3 RNA, with reduced Bcl-2 levels, in third-passage mES. Mbd3 knockdown resulted in partially reduced Bax, Bim, Trail, and caspase 3 RNA levels, unchanged Fasl levels, and increased Bcl-2 levels. The data are represented as mean ± SD (n=3). The letters a, b, c indicate significant differences among the groups (P<0.05).

Article Snippet: Mbd3 overexpression in mES The Mbd3 overexpression plasmids, FUW-Mbd3 (#52356) and control FUW-M2rtTA (#20342), were purchased from Addgene.

Techniques: Knockdown, RNA Expression, Western Blot, Staining, Expressing